Journal article
Role of helicity in the nonenzymatic template-directed primer extension of DNA
Sung Joon Park, Kimberley Laura Callaghan, Amanda Vera Ellis
Organic and Biomolecular Chemistry | Royal Society of Chemistry | Published : 2023
DOI: 10.1039/d3ob01179h
Abstract
Complexing a DNA primer with an RNA template showed improved nonenzymatic template-directed primer extension, attributed to a shift in the DNA helicity from a B-type towards an A-type helix. A 2-fold (deoxyadenosine) and 4.5-fold (deoxycytidine) increase in conversion from initial DNA primer to a primer + 1 nucleotide product was observed.
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Grants
Awarded by Australian Research Council Discovery program
Funding Acknowledgements
The authors would like to thank the Australian Commonwealth Government for funding through the Australian Research Council Discovery program (DP190100055) and Australian Research Training Program Scholarship. This work was performed in part at the Melbourne Protein Characterisation (MPC) at the Bio21 Institute, University of Melbourne.